In order to estimate the distribution of some polymorphisms for the CCR5, CCR 2, apoE, p53, ITGB3, and HFE genes in Russian long-livers from Western Siberia, a sample of 271 individuals (range 90-105 years) was examined. Ivanoshchuk, D E MikhaÄlova, S V Kulikov, I V Maksimov, V N Voevoda, M I Romashchenko, A G Our results suggest that the rs5918 (PlA 1/A 2) polymorphism in the ITGB3 gene may contribute to the susceptibility of sporadic Iranian coronary artery disease (CAD) patients. Conclusions Our T-ARMS-PCR in comparison with RFLP and allele-specific PCR is more advantageous because this PCR method allows the evaluation of both the wild type and the mutant allele in the same tube. The multiple logistic regression analysis showed a significant association of the rs5918 polymorphism and CAD according to dominant and recessive models (dominant model OR: 2.40, 95% CI: 1.33–4.35 p = 0.003, recessive model OR: 4.71, 95% CI: 1.32–16.80 p = 0.0067).
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Results Full accordance was found for genotype determination by the PCR-RFLP method.
Material and methods We set up T-ARMS-PCR for the rs5918 SNP in a single-step PCR and the results were validated by the PCR-RFLP method in 132 coronary artery disease (CAD) patients and 122 unrelated healthy individuals. Here, we present a newly designed multiplex tetra-primer amplification refractory mutation system – polymerase chain reaction (T-ARMS-PCR) for genotyping a single nucleotide polymorphism (SNP) (dbSNP ID: rs5918) in the human ITGB3 gene. Introduction The T to C transition at nucleotide 1565 of the human glycoprotein IIIa ( ITGB3) gene represents a genetic polymorphism (PlA 1/A 2) that can influence both platelet activation and aggregation and that has been associated with many types of disease.
Heidari, Mohammad Mehdi Soheilyfar, Sorour Common rs5918 (PlA 1/A 2) polymorphism in the ITGB3 gene and risk of coronary artery disease